Before the bacteria Collection Lab I made myself a hypothesis thinking Letter D (Jason's nose) will result in the biggest growth in bacteria. The reason on why I thought Jason's nose will have the biggest growth was because at that time jason was sick and had a runny nose. The other places I had swabbed around the school to see the growth was A: Mr.Ross's beared, B: Mr.Pavilc's hair, C: The inside of Mr.Shank's ear and D: The inside of Jason's nose.
Purpose: The purpose of this lab was to help us understand gram positive and negative bacteria and how they are very common all around us.
Materials:
- Red or black pencil crayon
- Agar
- petri dish
- 2 cotton swabs
- Surfaces to swab bacteria
- Incubator
- Masking tape
Method:
1. You will need to grab a petri dish and 2 cotton swab but DO NOT open the petri dish it until you have the surface you wanted to swab.
2. Once you have the petri dish make sure you put the petri dish upside down and label and divide the dish into 4 ( A>B>C>D). If you labe the top the lid, the lid will slide around and you will loose track on which bacteria was which.
3. Once you labeled the petri dish you may walk around the property until you have found a place you wanted to swab
4. Once you found the place carefully swab the area on just one of the cotton swab tip. You will need the other 3 tips for the rest of your groups.
5.once you carefully swabbed the area open the petri dish very carefully. Very gently move your cotton swab up and down while twirling your cotton swap with the tip thats infected around the divided group A
6.repeat steps 3 to 5 but will different surfaces and different divided areas.
7. Once you got all the bacteria on the petri dish carefully place it into the incubator for 2-3 days.
8. Pull out the bacteria and make sure to plug your nose because those bacteria are extremely stinky.
9. Record and take pictures of the bacteria.
10. Once you recorded the bacteria place the petri dish back into the incubator and come back to it in about 6-7 days.
11. After 6-7 days make yourself a conclusion on the growth of the 4 colonies.
Conclusion: My result did not support my hypothesis since the biggest growth in the bacteria was acually B(Mr:pavilc's hair). I think i should have gotten 4 totally different places to swab the bacteria, but i had swabbed for A and B hair on a human's body and C and D was the inside of the ear and nose of the human's body. Since I had swabbed similar places A and B had similar looking colonies and C and D also had similar colonies of bacteria. I feel like if I wanted to see if a sick person's runny nose have the biggest growth I should have gotten someone else's nose who weren't sick and see if there was any difference.
Purpose: The purpose of this lab was to help us understand gram positive and negative bacteria and how they are very common all around us.
Materials:
- Red or black pencil crayon
- Agar
- petri dish
- 2 cotton swabs
- Surfaces to swab bacteria
- Incubator
- Masking tape
Method:
1. You will need to grab a petri dish and 2 cotton swab but DO NOT open the petri dish it until you have the surface you wanted to swab.
2. Once you have the petri dish make sure you put the petri dish upside down and label and divide the dish into 4 ( A>B>C>D). If you labe the top the lid, the lid will slide around and you will loose track on which bacteria was which.
3. Once you labeled the petri dish you may walk around the property until you have found a place you wanted to swab
4. Once you found the place carefully swab the area on just one of the cotton swab tip. You will need the other 3 tips for the rest of your groups.
5.once you carefully swabbed the area open the petri dish very carefully. Very gently move your cotton swab up and down while twirling your cotton swap with the tip thats infected around the divided group A
6.repeat steps 3 to 5 but will different surfaces and different divided areas.
7. Once you got all the bacteria on the petri dish carefully place it into the incubator for 2-3 days.
8. Pull out the bacteria and make sure to plug your nose because those bacteria are extremely stinky.
9. Record and take pictures of the bacteria.
10. Once you recorded the bacteria place the petri dish back into the incubator and come back to it in about 6-7 days.
11. After 6-7 days make yourself a conclusion on the growth of the 4 colonies.
Conclusion: My result did not support my hypothesis since the biggest growth in the bacteria was acually B(Mr:pavilc's hair). I think i should have gotten 4 totally different places to swab the bacteria, but i had swabbed for A and B hair on a human's body and C and D was the inside of the ear and nose of the human's body. Since I had swabbed similar places A and B had similar looking colonies and C and D also had similar colonies of bacteria. I feel like if I wanted to see if a sick person's runny nose have the biggest growth I should have gotten someone else's nose who weren't sick and see if there was any difference.
DAY 3
DAY 7
For the second part of the lab we did gram staining to see if the bacteria was gram positive or gram negative. I have pictures of the lab which is uploaded right below.
If you see very closely at the image right below you have see a diplococcus right by the needle.
After gram staining we had mixed the coloney with the biggest growth and mixed it with some sterilized water and poured it onto another clean petri dish that contains agar. we divided it into 4 colonies onces again and labled( A>B>C>D). We got 4 different cleaning products and placed the petri dish back into the incubator to see which cleaning product had the biggest impact,
Analysis:
1. The bacteria was all evenly spaced out like a lawn of bacteria
2. Gives carbon and energy to the bacteria as well as nutrients since the bacteria was chemotrophic and heterotrophic. The bacteria wouldn't have been able to survive since the bateria is incapable of making their own food or light for energy.
3. Bleach was the most effective against the bacterial growth since it had the biggest zone of inhibition diameter of about 40mm.
4. The spray cleaner and the dish soap was tie in last since the bacteria was able to grow around the area with no zone of inhibition diameter. It did truly suprize me since we use dish soap to clean out dishes and it wasn't able to kill the simpliest bacteria that was in hair. Also how the spray cleaner is even called a cleaner but wasn't able to do any damage to the bacteria. My hypothesis on this was since we use dish soap and dish cleaner daily, maybe the bacteria's were able to be immuned to those cleaners since it is used in out daily lifes.
5. The experiment control in this lab would be the cleaners we have chosen since we are able to change the cleaners. The cleaner results will depend on the outcome of the bacteria which we cannot control.
6. I think since we put a very small amount of the cleaner made an impact. The ratio of the cleaners and bacteria would be about 4:10. Since there was more bacteria it was an unfair experiment for the cleaners. if we had put more cleaners and matched the ratios I think even the worst cleaners would be able to give a bigger inhibition area.
7. The bacteria that is able to be immune to that cleaning product will survive longer than those who can't. Since they were immuned and has a bigger chance on surviving they are able to reproduce alot more than those who aren't immuned passing on their immuned genes to future generations. After a couple generation majority of that bateria will be immuned to that cleaning product.
8. Mr Pavlic's hair had the biggest growth.
1. The bacteria was all evenly spaced out like a lawn of bacteria
2. Gives carbon and energy to the bacteria as well as nutrients since the bacteria was chemotrophic and heterotrophic. The bacteria wouldn't have been able to survive since the bateria is incapable of making their own food or light for energy.
3. Bleach was the most effective against the bacterial growth since it had the biggest zone of inhibition diameter of about 40mm.
4. The spray cleaner and the dish soap was tie in last since the bacteria was able to grow around the area with no zone of inhibition diameter. It did truly suprize me since we use dish soap to clean out dishes and it wasn't able to kill the simpliest bacteria that was in hair. Also how the spray cleaner is even called a cleaner but wasn't able to do any damage to the bacteria. My hypothesis on this was since we use dish soap and dish cleaner daily, maybe the bacteria's were able to be immuned to those cleaners since it is used in out daily lifes.
5. The experiment control in this lab would be the cleaners we have chosen since we are able to change the cleaners. The cleaner results will depend on the outcome of the bacteria which we cannot control.
6. I think since we put a very small amount of the cleaner made an impact. The ratio of the cleaners and bacteria would be about 4:10. Since there was more bacteria it was an unfair experiment for the cleaners. if we had put more cleaners and matched the ratios I think even the worst cleaners would be able to give a bigger inhibition area.
7. The bacteria that is able to be immune to that cleaning product will survive longer than those who can't. Since they were immuned and has a bigger chance on surviving they are able to reproduce alot more than those who aren't immuned passing on their immuned genes to future generations. After a couple generation majority of that bateria will be immuned to that cleaning product.
8. Mr Pavlic's hair had the biggest growth.